Product Details
- Verified Reactivity
- All species (6x His tag epitope)
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- KLH-conjugated His-tag peptide
- Formulation
- This antibody is provided in 50% glycerol in aqueous buffered solution with preservatives.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light. Avoid exposure to sodium azide.
- Application
-
ELISA - Quality tested
WB - Verified - Recommended Usage
Each lot of this antibody is quality control tested by direct ELISA assay. For direct ELISA, a concentration range of 0.01 - 1.0 ?g per ml is recommended. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 ?g per ml. Avoid using diluent solutions containing sodium azide. Sodium azide is an inhibitor of horseradish peroxidase. It is recommended that the end-user titrate each lot of reagents for optimal performance.
- Application References
(PubMed link indicates BioLegend citation) -
- Burritt JB, et al. 1995. J. Biol. Chem. 270:16974.
- Taylor RM, et al. 2007. Methods Mol. Biol. 412:429.
- Product Citations
-
- RRID
- AB_2734520 (BioLegend Cat. No. 652503) AB_2563555 (BioLegend Cat. No. 652504)
Antigen Details
- Function
- Recombinant poly-histidine tag detection
- Biology Area
- Cell Biology
- Gene ID
- NA
- UniProt
- View information about His Tag on UniProt.org
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