Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse EPO
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 ?g per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This product does not cross react with human EPO.
- RRID
- AB_2563470 (BioLegend Cat. No. 660302)
Antigen Details
- Structure
- 166 amino acid, predicted molecular weight of approximately 18.6 kD, migrates at approximately 30-34 kD as a result of its glycosylation.
- Distribution
-
EPO is primarily expressed in the kidneys (70-90% of the total EPO) and liver. EPO is expressed by hepatocytes, cortical interstitial fibroblasts in ischemic kidneys, astrocytes, and neurons under hypoxia.
- Function
- EPO is the major regulator of differentiation, proliferation, and survival of erythroid progenitors. EPO expression is induced by hypoxia, HIF, and other transcription factors. EPO has a possible role in neuroprotection.
- Interaction
- Erythroid progenitor cells.
- Ligand/Receptor
- EpoR (homodimer).
- Cell Type
- Fibroblasts, Neurons
- Biology Area
- Cell Biology
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Shoemaker CB, et al. 1986. Mol. Cell. Biol. 6:849.
2. McDonald JD, et al. 1986. Mol. Cell. Biol. 6:842.
3. Chin K, et al. 2000. Brain Res. Mol. Brain Res. 81:29.
4. Snow JW, et al. 2009. J. Biol. Chem. 284:29310.
5. Kassouf MT, et al. 2010. Genome Res. 2010:20:1064.
6. Paliege A, et al. 2010. Kidney Int. 77:312.
7. Sinclair AM, et al. 2010. Blood 115:4264.
8. Alnaeeli M, et al. 2012. Anat. Res. Int. 2012:953264. - Gene ID
- 2056 View all products for this Gene ID
- UniProt
- View information about EPO on UniProt.org
