Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant human NGAL
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
ELISA Detection - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by ELISA. For ELISA detection applications, a concentration of 0.15 ?g/mL is recommended. To obtain a linear standard curve, serial dilutions of NGAL recombinant protein ranging from 2500 to 39.1 pg/mL is recommended for each ELISA plate.
It is recommended that the reagent be titrated for optimal performance.
- Application Notes
ELISA Detection: The biotinylated M049D6 antibody is useful as a detection antibody for a sandwich ELISA assay, when used in conjunction with purified M0415B7 (Cat. No. 661902) antibody as the capture antibody.
Note: For testing human NGAL in cell culture supernatant, serum, plasma, urine, saliva, and other biological fluids, BioLegend's LEGEND MAX? Kits (Cat. No. 443407 & 443408) are specially developed and recommended.
- Application References
(PubMed link indicates BioLegend citation) -
- Iqbal N, et al. 2013. Expert Opin. Med Diagn. 7:209.
- Singer E, et al. 2013. Acta. Physiol. (Oxf) 207:663.
- Palazzuoli A, et al. 2012. Clin. Chim. Acta. 413:1832.
- Flo TH, et al. 2004. Nature 432:917
- Goetz DH, et al. 2002. Mol. Cell. 10:1033.
- Leung L, et al. 2012. PLoS One 7:e46677
- Kjeldsen L, et al. 1993. J. Biol. Chem. 268:10425.
- RRID
- AB_2650710 (BioLegend Cat. No. 503304)
Antigen Details
- Structure
- 193 amino acid monomer with a predicted molecular weight of approximately 22 kD.
- Distribution
-
Secreted by epithelial cells, macrophages, neutrophils. Increased levels have been observed in plasma, serum, and urine in various conditions, such as metastatic breast and colorectal cancer, acute kidney injury, pancreatitis and preeclampsia.
- Function
- Apoptosis, innate immunity, renal development.
- Interaction
- Disulfide-linked with MMP9
- Ligand/Receptor
- SLC22A17 (24p3R)
- Cell Type
- Epithelial cells, Macrophages, Neutrophils
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Innate Immunity, Signal Transduction
- Gene ID
- 3934 View all products for this Gene ID
- UniProt
- View information about NGAL on UniProt.org
