Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- In-house recombinant mouse PCSK9
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ELISA Capture - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by ELISA. For ELISA capture applications, a concentration of 2 ?g/mL is recommended. To obtain a linear standard curve, serial dilutions of PCSK9 recombinant protein ranging from 4000 to 62.5 pg/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance.
- Application Notes
ELISA Capture: The purified M114G10 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated Poly5251 antibody as the detecting antibody.
Note: For testing mouse PCSK9 in serum, plasma, cell culture supernatant, cell lysate, tissue homogenate, and other biological fluids, BioLegend's LEGEND MAX? Kits (Cat. No. 443207 & 443208) are specially developed and recommended.
- RRID
- AB_2686955 (BioLegend Cat. No. 532402)
Antigen Details
- Structure
- Monomer. The molecular mass of a full-length and mature protein is 74.8 kD and 53.7 kD, respectively.
- Distribution
-
Hepatocytes, renal cells, neuroepithelioma, and Schwann cells.
- Function
- PCSK9 regulates LDLR, VLDLR, APOER, and APOER2 levels, and therefore, LDLc levels. PCSK9 is regulated by SREBP-2.
- Interaction
- Hepatocytes.
- Ligand/Receptor
- LDLR, VLDLR, APOB, LRP1, and LRP8.
- Biology Area
- Cell Biology, Neuroinflammation, Neuroscience, Signal Transduction
- Molecular Family
- Enzymes and Regulators
- Antigen References
-
1. Seidah NG, et al. 2003. Proc. Natl. Acad. Sci. USA 100:928.
2. Naureckiene S, et al. 2003. Arch. Biochem. Biophys. 420:55.
3. Costet P, et al. 2008. Trends Biochem. Sci. 33:426.
4. Grefhorst A, et. al. 2008. J. Lipid Res. 49:1303.
5. Tavori H, et al. 2013. Circulation 127:2403.
6. Denis M, et al. 2012. Circulation 125:894.
7. Tibolla G, et al. 2011. Nutr. Metab. Cardiovasc. Dis. 21:835. - Gene ID
- 100102 View all products for this Gene ID
- UniProt
- View information about PCSK9 on UniProt.org
