Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- In-house recombinant mouse MIF.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ELISA Capture - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration of 3.0 ?g/mL is recommended. To obtain a linear standard curve, serial dilutions of MIF recombinant protein ranging from 10 to 0.16 ng/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance.
- Application Notes
ELISA Capture: The purified M3907B02 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated Poly5240 antibody (Cat. No. 524004) as the detecting antibody.
Note: For testing mouse MIF in serum, plasma, cell culture supernatant, and cell lysate, BioLegend's LEGEND MAX? Kits are specially developed and recommended.- RRID
- AB_2686958 (BioLegend Cat. No. 532502)
Antigen Details
- Structure
- 115 amino acids with a predicted molecular weight of approximately 12.5 kD.
- Distribution
-
Activated T-cells, macrophages, eosinophils, epithelial cells, and endothelial cells.
- Function
- MIF has been associated with a clear pro-inflammatory and proatherogenic role in cardiovascular diseases. MIF also plays important roles in adipose tissue inflammation and innate and acquired immunity.
- Interaction
- Monocytes and macrophages, mesenchymal cells, and B-cells.
- Ligand/Receptor
- CD44/CD74, CXCR4, CXCR2, and CXCR7.
- Cell Type
- T cells, Macrophages, Eosinophils, Epithelial cells, Endothelial cells
- Biology Area
- Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Innate Immunity, Signal Transduction
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Zhou H, et al. 2009. PLoS One. 4:e6087.
2. Potolicchio I, et al. 2003. J. Biol. Chem. 278:30889.
3. Meyer-Siegler K and Iczkowski K. 2005. BMC Cancer 5:73.
4. Babu SN, et al. 2012. Asian Pac. J. Cancer Prev. 13:1737.
5. van der Vorst EP, et al. 2015. Front Immunol. 6:373.
6. Kim BS, et al. 2015. Exp. Mol. Med. 47:e161.
7. Sanchez-Zamora YI, et al. 2014. J. Diabetes Res. 2014:804519.
8. Gilliver SC, et al. 2011. Exp. Dermatol. 20:1.
9. Bach JP, et al. 2008. Oncology 75:127. - Gene ID
- 17319 View all products for this Gene ID
- UniProt
- View information about MIF on UniProt.org
