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產(chǎn)品編號	bs-0535R
英文名稱	Rabbit　Anti-TFF3　antibody
中文名稱	三葉肽因子3抗體
別　　　　名	Trefoil Factor 3; Trefoil factor3; hP1.B; Intestinal trefoil factor; ITF; mITF; OTTMUSP00000021729; Polypeptide P1.B; TFF3; TFI; TREFOIL; Trefoil factor (intestinal).
	Specific References　　(2)　　　　　\|　　　　　bs-0535R has been referenced in 2 publications. [IF=4.36]　Jiacheng Li. et al. Enhanced therapeutic effect on colitis with powder formulations of Painong San associated with the promotion of intestinal adhesion and absorption. J Ethnopharmacol. 2022 May;289:115030　　IHC　;　　Mouse.　　 PubMed:35093456 [IF=1.966]　Wadey et al. Tissue expression and correlation of a panel of urinary biomarkers following cisplatin-induced kidney injury. (2013) Toxicol.Pathol. 42:591-602　　IHC　;　　Human,?Mouse,?Rat,?Cow.　　 PubMed:23823703
研究領(lǐng)域	信號轉(zhuǎn)導(dǎo)　　細(xì)胞凋亡
抗體來源	Rabbit
克隆類型	Polyclonal
交叉反應(yīng)	Rat,Mouse,Human (predicted: Cow)
產(chǎn)品應(yīng)用	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
理論分子量	6.5kDa
細(xì)胞定位	細(xì)胞漿　細(xì)胞外基質(zhì)　分泌型蛋白
性　　　　狀	Liquid
濃　　　　度	1mg/ml
免　疫　原	KLH conjugated synthetic peptide derived from human TFF3:　51-80/94
亞　　　　型	IgG
純化方法	affinity purified by Protein A
緩　沖　液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng)	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
產(chǎn)品介紹	Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium. This gene is expressed in goblet cells of the intestines and colon. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq, Jul 2008] Subunit: Monomer. Homodimer; disulfide-linked. Subcellular Location: Secreted, extracellular space, extracellular matrix, apical lamina. Tissue Specificity: Expressed in goblet cells of the intestines and colon (at protein level). Expressed by goblet cells of small and large intestinal epithelia and also by the uterus. Similarity: Contains 1 P-type (trefoil) domain. SWISS: Q07654 Gene ID: 7033 Database links: Entrez Gene: 7033 Human Omim: 600633 Human SwissProt: Q07654 Human Unigene: 82961 Human 三葉草肽Ⅲ是三葉肽中結(jié)構(gòu)獨(dú)特的一類小分子肽,它對于胃黏膜保護(hù)及損傷后修復(fù)具有重要作用,它的作用機(jī)制復(fù)雜,通過與其受體作用機(jī)制及信號傳導(dǎo)機(jī)制的研究,以加深其對胃黏膜保護(hù)機(jī)制的認(rèn)識。在促進(jìn)黏膜愈合的治療上,三葉肽是一個新的思路。局部應(yīng)用三葉肽可能使黏膜凝膠產(chǎn)生更大的穩(wěn)定性,使胃黏膜對各種損傷因子的防御能力增強(qiáng)。三葉肽在誘導(dǎo)上皮修復(fù)上的直接作用,可用于許多胃腸道疾病。它作為輔助劑或取代皮質(zhì)類固醇或其他免疫抑制劑，在潰瘍病及炎癥性腸病的治療上可能是一個突破。三葉肽研究可能對胃十二指腸潰瘍和炎癥、壞死性小腸結(jié)腸炎、放療和化療導(dǎo)致的黏膜炎帶來新的治療方法。三葉草肽Ⅲ與黏液糖蛋白相互作用,可誘導(dǎo)上皮細(xì)胞遷移，參與信號傳導(dǎo)機(jī)制，調(diào)節(jié)胃酸及運(yùn)動功能。
產(chǎn)品圖片	Paraformaldehyde-fixed, paraffin embedded (Mouse small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TFF3 ) Polyclonal Antibody, Unconjugated (bs-0535R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TFF3) Polyclonal Antibody, Unconjugated (bs-0535R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TFF3) Polyclonal Antibody, Unconjugated (bs-0535R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TFF3) Polyclonal Antibody, Unconjugated (bs-0535R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TFF3) Polyclonal Antibody, Unconjugated (bs-0535R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: rat colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-TFF3 Polyclonal Antibody, Unconjugated(bs-0535R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: mouse small intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-TFF3 Polyclonal Antibody, Unconjugated(bs-0535R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Expressed by goblet cells of small and large intestinal epithelia and also by the uterus.