產(chǎn)品類型:標簽抗體
研究領域:腫瘤 細胞生物 免疫學 染色質和核信號 信號轉導 合成與降解 結合蛋白
抗體來源:Rabbit
克隆類型:Polyclonal
交叉反應:Recombinant protein
產(chǎn)品應用:WB=1:1000-5000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
性 狀:Liquid
濃 度:1mg/ml
免 疫 原:Synthetic peptide: HHHHHH conjugated to KLH
亞 型:IgG
純化方法:affinity purified by Protein A
緩 沖 液:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事項:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMedPubMed
產(chǎn)品介紹:The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
SWISS:
N/A
Gene ID:
N/A
標簽抗體
蛋白質純化的新途徑─應用His-Tag抗體檢測His-Tag融合蛋白
1) 6xHis與不同的Sepharose純化填料結合(捆綁),可不受構象的影響,在天然或變形的條件下可進行進一步純化。
2) 可在溫和的洗脫液環(huán)境中,其結合、洗滌及洗脫步驟不會對蛋白結構產(chǎn)生影響。
3) 6xHis標簽比普通(Tag)要小得多,經(jīng)純化后的蛋白可直接進行下游操作?捎糜谌魏伪磉_系統(tǒng)(包括:細菌,桿狀病毒和哺乳動物)
4) 6xHis標簽在生理溶液pH下不帶電荷,其標簽不會干涉重組蛋白的結構和功能, 6xHis不會影響蛋白分泌
5) 6xHis標簽免疫原性差,重組蛋白可以不必除去標簽而直接作為抗原進行免疫
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