Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- KLH conjugated Apo E peptide encompassing amino acids 154-162.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
Direct ELISA, IHC-P - Verified - Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 1.0 ?g per mL.?For Direct ELISA applications, a concentration range of 0.004 - 0.4 ?g/mL is recommended.?For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 5.0 ?g/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This antibody cross-reacts with Apo E2, Apo E3, and Apo E4.
- RRID
- AB_2728599 (BioLegend Cat. No. 852801) AB_2728600 (BioLegend Cat. No. 852802)
Antigen Details
- Structure
- Human Apo E is a 299 amino acid protein with a molecular mass of 32 kD, and exists as 3 major alleles, Apo E2, Apo E3, and Apo E4.
- Distribution
-
Tissue sources: Ubiquitously expressed. Highest levels in liver, brain, spleen, adrenal gland, and kidney.
Cellular distribution: Plasma membrane, nucleus, endoplasmic reticulum, golgi apparatus, endosome, and extracellular. - Function
- Apo E is involved in lipoprotein metabolism as well as the transport of fat-soluble vitamins, and cholesterol. Apo E also forms complexes with amyloid beta peptides leading to its uptake and degradation by microglia.
- Interaction
- Tau, amyloid beta
- Ligand/Receptor
- LDL receptor, SORL1.
- Biology Area
- Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
- Molecular Family
- Apolipoproteins
- Antigen References
-
- Mahley RW. 2016. Arterioscler Thromb Vasc Biol. 36: 1305-1315.
- Riedel BC, et al. 2016 J Steroid Biochem Mol Biol. 160:134-47.
- Tai LM, et al. 2016 Acta Neuropathol. 131(5):709-23.
- Kim J, et al. 2014. Mol. Cells. 37:767
- Holtzman DM, et al. 2012. Cold Spring Harb. Perspect. Med. 2:a006312
- Gene ID
- 348 View all products for this Gene ID
- UniProt
- View information about Apo E, 154-162 on UniProt.org
