Product Details
- Verified Reactivity
- SARS-CoV-2
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Partial recombinant protein corresponding to the S1 subunit of SARS-CoV-2 S protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ELISA - Verfied - Recommended Usage
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25?- 1.0??g/mL. For ELISA applications, a concentration of 65.4?ng/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
BioLegend offers multiple clones that recognize SARS-CoV-2 S protein S1. Clone A20103H displayed the strongest performance for western blot of all clones validated for the application.
- RRID
- AB_2890872 (BioLegend Cat. No. 944401) AB_2890872 (BioLegend Cat. No. 944402)
Antigen Details
- Structure
- Spike glycoprotein is a homotrimer. Each monomer consists of 1,273 a.a with a molecular weight of approximately 141 kD and consists of S1 and S2 subunits. S1 subunit is 673 a.a. long.
- Distribution
-
Viral envelope protein, host cell membrane, host cell endoplasmic reticulum-Golgi intermediate compartment membrane
- Function
- Host cell surface receptor binding
- Interaction
- ACE2
- Ligand/Receptor
- ACE2
- Biology Area
- COVID-19
- Antigen References
-
- Walls AC, et al. 2020. Cell. 181:281-292.
- Yan R, et al. 2020. Science. 367:1444-1448.
- Wrapp D, et al. 2020. Science. 367:1260-1263.
- Shang J, et al. 2020. Proc Natl Acad Sci U S A. 117:11727-11734.
- Gene ID
- 43740568 View all products for this Gene ID
- UniProt
- View information about Viral Protein on UniProt.org
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