Product Details
- Verified Reactivity
- SARS-CoV-2
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Partial recombinant SARS-CoV-2 S protein corresponding to S1 subunit
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
Direct ELISA, FC - Verified - Recommended Usage
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 1.0 ?g/mL. For?Direct ELISA, a concentration of 114.9 ng/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5??g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
BioLegend offers multiple clones that recognize SARS-CoV-2 S protein S1. Clone A20103H displayed the strongest performance for western blot of all clones validated for the application.
- Product Citations
-
- RRID
- AB_2890875 (BioLegend Cat. No. 945101) AB_2890875 (BioLegend Cat. No. 945102)
Antigen Details
- Structure
- Spike glycoprotein is a homotrimer. Each monomer consists of 1,273 amino acids with a theoretical molecular weight of 141 kD, and consists of S1 and S2 subunits.
- Distribution
-
Viral envelope protein, host cell membrane, host cell endoplasmic reticulum-Golgi intermediate compartment membrane
- Function
- Mediates fusion between virus and host cell membranes
- Interaction
- ACE2
- Ligand/Receptor
- ACE2
- Biology Area
- COVID-19
- Antigen References
-
1. Walls AC, et al. 2020. Cell. 181(2):281-292.
2. Yan R, et al. 2020. Science. 367 (6485):1444-1448.
3. Wrapp D, et al. 2020. Science. 367 (6483):1260-1263.
4. Shang J, et al. 2020. PNAS. 117(21):11727-11734 - Gene ID
- 43740568 View all products for this Gene ID
- UniProt
- View information about Viral Protein on UniProt.org
